You are a senior microbiologist studying mycobacteria. Please find me a few copies of the protocol of directly scratching the board to pick a single clone from the frozen Mycobacterium smegmatis ms2155 mixed with glycerol
您是一位研究分枝杆菌的高级微生物学家。请给我找几份直接刮板的协议，从与甘油混合的冷冻耻垢分枝杆菌 ms2155 中挑选一个克隆

Protocol for Picking a Single Clone of Mycobacterium smegmatis mc2155
选择耻垢分枝杆菌 mc2155 单克隆的方案

• Frozen stock of M. smegmatis mc2155 in glycerol
  M. smegmatis mc2155 甘油冷冻原液
• Sterile inoculating loop or toothpick
  无菌接种环或牙签
• LB agar or 7H10 agar plates
  LB琼脂或7H10琼脂平板
• Incubator set to 37°C
  培养箱设置为 37°C
• Sterile saline or broth for resuspension (optional)
  用于重悬的无菌生理盐水或肉汤（可选）

1. Thaw the Frozen Stock:
   • Remove the frozen stock of M. smegmatis mc2155 from the -80°C freezer and allow it to thaw on ice for a few minutes.
     从 -80°C 冰箱中取出 M. smegmatis mc2155 的冷冻原液，让它在冰上解冻几分钟。
2. Prepare the Agar Plate:
   • Label an LB or 7H10 agar plate (with appropriate supplements if needed) and allow it to warm to room temperature if it was stored in the refrigerator.
     标记 LB 或 7H10 琼脂平板（如果需要，添加适当的补充剂），如果储存在冰箱中，则让它加热至室温。
3. Scrape the Frozen Stock:
   • Using a sterile inoculating loop or toothpick, gently scrape the surface of the frozen stock. Ensure you only pick a small amount to avoid contamination with multiple clones.
     使用无菌接种环或牙签，轻轻刮擦冷冻原液的表面。确保只选择少量，以避免被多个克隆污染。
4. Inoculate the Agar Plate:
   • Streak the scraped material onto the surface of the agar plate in a zig-zag pattern to allow for isolation of individual colonies.
     将刮下的材料以锯齿形图案划到琼脂平板的表面上，以允许分离单个菌落。
5. Incubate:
   • Incubate the agar plate upside down at 37°C for 24-48 hours, or until colonies are visible.
     将琼脂平板在 37°C 下倒置孵育 24-48 小时，或直到看到菌落。
6. Select a Single Colony:
   • After incubation, identify a well-isolated colony. Using a sterile inoculating loop, pick the colony and transfer it to a fresh agar plate or liquid medium for further culturing.
     孵育后，确定一个分离良好的菌落。使用无菌接种环，选择菌落并将其转移到新鲜琼脂平板或液体培养基中进行进一步培养。
7. Storage:
   • If you need to store the selected clone, you can mix it with glycerol and freeze it at -80°C for long-term storage.
     如果需要储存选定的克隆，可以将其与甘油混合，并在-80°C下冷冻以进行长期储存。